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qEVoriginal / 35nm Legacy - 5 Pack

US $229

The qEVoriginal / 35 nm Gen 2 column is now available here,

The qEVorignal Legacy is also available in the 70 nm+ series, this 35 nm+ column has an optimal recovery range of 35 nm to 350 nm.

Our most popular qEV column size, the qEVoriginal / 35 nm Legacy contains a resin with an approximately 35 nm pore size. Proteins and other contaminating molecules smaller than 35 nm enter the pores of the resin and are delayed in their passage through the column, eluting in later fractions. The use of qEV columns leads to higher quality Exosome measurements and analysis when combined with TRPS measurements.

Removal of proteins

Under ideal conditions, SEC purification of vesicles is able to remove >99% of contaminating free proteins.

Removal of lipids

A significant problem with ultracentrifugation is that the similar density of HDL and LDL particles to Exosomes means that the particles often co-isolate with Exosomes. The collection of appropriate fractions from qEVoriginal Legacy separation results in up to 95% removal of contaminating HDL from Exosome samples.

Reproducibility of purification

qEVoriginal Legacy columns have undergone extensive research and development to ensure that the separation does not introduce any bias into the sample.


qEVoriginal Legacy columns may be used up to 5 times, with a cleaning step in between uses, making their use very cost effective compared to all other separation options.

qEVoriginal Legacy Size

qEVoriginals are presently 10 ml volume, targeted at 500 μL of loading volume. 

Quality control

All of our qEV columns are certified to ISO 13485 (2016).

For a proper comparison of the qEV columns available, please visit the qEV Isolation Page.

The new qEVoriginal / 35 nm Gen 2 column is now available here,

Product Code: SP5

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